Isolation of high-purity viral RNA from human swab solution is essential for clinical diagnosis and molecular research. A1 Viral RNA Isolation Kit provides a rapid and simple method for isolation of highly pure viral RNA from virus-containing cell-free body fluids.

The enzyme-free protocol can be completed in 20-25 minutes. As specifically optimized lysis/washing solutions and silica-based spin columns designed to capture exclusively viral RNAs are employed, high efficiency and purity are achieved by the end of the isolation process. A1 Viral RNA Isolation Kit is available in two different packaging sizes; 50 tests or 100 tests.

150 μl serum or plasma sample is sufficient for a single test. Due to the simple procedure of the kit that requires only basic laboratory equipment, it is possible to isolate viral RNA from various samples simultaneously. The recovered viral RNA can be used directly in downstream applications such as RT-PCR, qRT-PCR, qPCR, viral detection, viral load determination, and viral genotyping.

The procedure is simply as follows; under highly denaturing conditions lysis and inactivation of RNases is accomplished in serum and plasma samples. RNA Carrier is added at the start serving to enhance the binding affinity of viral RNAs to the columns and to decrease the degradation possibility of viral RNAs by remaining RNases.

After completion of lysis, the free viral RNA is transferred into the columns and the silica gel-based membrane captures the viral RNAs selectively. In the following consecutive washing steps, contaminants such as proteins, nucleases, or inhibitors are efficiently washed away and highly pure viral RNA is obtained.